Promega
Lumit™ Immunoassay Labeling Kit and Detection Reagents

Lumit™ Immunoassays are based on NanoLuc® Binary (NanoBiT®) technology. The NanoBiT® system is composed of two subunits, Large BiT (LgBiT) and Small BiT (SmBiT), that have been optimized for stability and minimal self-association. In Lumit™ Immunoassays, antibodies are chemically labeled with SmBiT and LgBiT. Upon binding labeled antibodies to an analyte, the SmBiT and LgBiT subunits come in close proximity, reassemble into a functional NanoLuc® luciferase enzyme to generate a luminescent signal in the presence of substrate. Use the Lumit™ Immunoassay Labeling Kit to label your antibodies of choice and build your custom Lumit™ Immunoassay using Lumit™ Immunoassay Detection Reagents.