Scientists conducting the process of Polymerase chain reaction (PCR) to amplify DNA by using a thermocycler to create samples
[Source: Monty Rakusen/Getty Images]

Promega has released XpressAmp direct amplification reagents that facilitate RNA extraction-free sample preparation. The new automation friendly reagents allow laboratories testing for COVID-19 have a tool that allows them to skip the RNA extraction step of the many testing workflows and move directly to PCR amplification.

The direct amplification workflow involves three key steps: collection and storage of the samples of nasopharyngeal swabs in transport media; sample mixing using XpressAmp Lysis Buffer (1:1) and incubate for 10 minutes at room temperature; and add lysed sample to RT-qPCR containing XpressAmp solution.

Viral inactivation studies performed on behalf of Promega by Dr. Thomas Klimkait from the Molecular Virology Group, Department of Biomedicine, University of Basel, Switzerland, found that the XpressAmp Lysis Buffer successfully inactivated the SARS-CoV-2 virus.

Promega Corp.

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