According to a new study from the Yale University School of Medicine, genotoxic metabolites present in patients with inflammatory bowel disease (IBD) may play a role in the development of colorectal cancer.
The authors of the study, published in the journal Science, discovered a family of genotoxins called indolimines, that are produced by Morganella morganii—a bacterial species linked to colorectal cancer. The researchers showed that indolimines can generate double-stranded breaks in DNA, in both cell-free and cell-based DNA damage assays. Such double-strand DNA damage could trigger the development of cancer.
“The study of Cao et al. reveals that the human colon microbiota—already highly implicated in colorectal pathogenesis—has a broad-ranging, previously unimagined capacity to produce genotoxins with colon disease-inducing potential,” noted Jens Puschhof, PhD, a scientist at the Epithelium Microenvironment Interaction Laboratory (EMIL) that is part of the German Cancer Research Center in Heidelberg, Germany, and Cynthia Sears, PhD, the Bloomberg-Kimmel professor of cancer immunotherapy at Johns Hopkins, in a perspective article on the work, published in the same issue of Science. (Puschhof and Sears were not involved in the current study).
Earlier studies had shown that microbiota-derived genotoxic metabolites likely play a critical role in driving pathogenesis that leads to colorectal cancer, but the identity of the spectrum of genotoxic chemicals produced by commensal gut microbes in IBD was unknown.
In the current study, Yiyun Cao, a graduate student, and her colleagues in the laboratory led by Noah Palm, an associate professor of immunobiology at the Yale school of medicine, combined untargeted metabolomics and bioactivity-guided natural product discovery techniques to isolate and characterize indolimines.
They demonstrated that, in a mouse model of colon cancer, the presence of aat (aspartate aminotransferase) expressing Morganella morganii in the gut microbiota exacerbated tumor growth. However, a non-indolimine-producing mutant strain of the same bacterial species lacked this genotoxicity and did not promote tumor growth. This observation indicates that the production of indolimines is required to promote the growth of tumors in the colon when these bacteria are transplanted into a susceptible mouse model. The researchers have also identified other bacteria belong to the phyla Firmicutes and Actinobacteria that produce metabolites that are likely to be genotoxic.
“These studies reveal the existence of a previously unexplored universe of genotoxic small molecules from the microbiome that may affect host biology in homeostasis and disease,” the authors concluded. These findings can help identify novel genotoxicity mechanisms that trigger disease.