Designed specifically for detection of mutations from liquid biopsy samples, the Spotlight 59 Oncology Panel is a turnkey solution for sequencing, offering ultra-sensitive variant detection from low input quantities of DNA. The high sensitivity of Spotlight 59 makes it ideal for CTCs, cfDNA, and other challenging sample types such as FFPE. Combined with ERASE-Seq, a statistically powered variant caller, Spotlight 59 delivers 100% specificity at 0.2% allele frequency. This new panel offers comprehensive and hotspot coverage of 59 clinically relevant oncology-related genes, using a 277-amplicon design to generate multiple libraries compatible with Illumina sequencing platforms. The product is a complete kit that includes all elements necessary for generating ready-to-sequence libraries, including primer pairs and indexed sequencing adapters.