Clinical Samples in Flow Cytometry: Best Practices for Sample Preparation and Sample Choice

Originally Aired: May 2, 2023
Time: 8:00 am PT, 11:00 am ET, 17:00 CET

Whole blood sample stability as an analytical parameter should be assessed when developing pharmacodynamic flow cytometry assays. Sample stability will determine the robustness of detecting not only the markers of interest but also the cell populations of interest. Sample preparation which can improve sample stability is crucial for flow cytometry analysis in multi-center clinical trials as it allows for transport from clinical sites to the laboratory.

Various techniques such as fixative-containing direct blood draw collection tubes or freezing of whole blood at clinical sites with custom fixatives, these methods can increase the sample stability and storage. Based on the flow cytometry markers, fixatives can extend the sample stability several weeks to months. PBMC isolation can also be an alternative solution for high-quality flow cytometry data. This webinar explored several strategies for extending sample stability, alternatives to whole blood samples in flow cytometry, and their successful implementations in clinical studies.

What you will learn:

• Key considerations for sample preparation for high-quality flow cytometry data
• Advantages of using cryopreserved PBMCs vs. whole blood for flow cytometry
• Flow cytometry case studies in whole blood and PBMCs

A live Q&A session followed the presentation.